| Объем | 50 мкл |
| Синонимы | Eukaryotic translation initiation factor 3 subunit M (eIF3m) (Fetal lung protein B5) (hFL-B5) (PCI domain-containing protein 1), EIF3M, HFLB5 PCID1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q7L2H7 |
| Иммуноген | Recombinant Human Eukaryotic translation initiation factor 3 subunit M protein (1-374AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Eukaryotic translation initiation factor 3 subunit M |
| Области исследований | Microbiology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: EIF3M antibody at 2.02µg/ml
Lane 1: Hela whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: Mouse gonadal tissue
Lane 4: Human placenta tissue
Lane 5: Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43, 28 kDa
Observed band size: 43 kDa
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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA745333DSR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA745333DSR1HU at dilution of 1:100
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Immunoprecipitating EIF3M in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA745333DSR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA745333DSR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: EIF3M Antibody.