| Объем | 50 мкг |
| Синонимы | Eukaryotic initiation factor 4A-II (eIF-4A-II) (eIF4A-II) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-2), EIF4A2, DDX2B EIF4F |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14240 |
| Иммуноген | Recombinant Human Eukaryotic initiation factor 4A-II protein (1-407AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice, yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Eukaryotic initiation factor 4A-II antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 44.8 kDa
Observed band size: 44.8 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA00384A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA00384A0Rb at dilution of 1:100
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
All lanes: EIF4A2 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00384A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA00384A0Rb at dilution of 1:100
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Immunofluorescence staining of A549 cells with CSB-PA00384A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: EIF4A2 Antibody.