Объем | 100 мкл |
Синонимы | Eukaryotic translation initiation factor 3 subunit H (eIF3h) (Eukaryotic translation initiation factor 3 subunit 3) (eIF-3-gamma) (eIF3 p40 subunit), EIF3H, EIF3S3 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O15372 |
Иммуноген | Recombinant Human Eukaryotic translation initiation factor 3 subunit H protein (80-340AA) |
Источник | Rabbit |
Видовая специфичность | Human, Rat |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
Примечание | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: EIF3H antibody at 7µg/ml
Lane 1: NIH/3T3 whole cell lysate
Lane 2: Rat brain tissue
Lane 3: U251whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA007537ESR2HU at dilution of 1:100
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