EIF4G1 (Ab-1232) AntibodyСпецификация| Объем | 100 мкл | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q04637 | | Иммуноген | Peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G. | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | <p>eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3, in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis <br /><br />De Gregorio, E. et al. (1998) RNA 4, 828-836. <br />Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382. <br />Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136. <br />Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HepG2 and HT29 cells using eIF4G(Ab-1232) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G(Ab-1232) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using eIF4G(Ab-1232) Antibody.
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Наименование: EIF4G1 (Ab-1232) Antibody.