Объем | 50 мкл |
Синонимы | Eukaryotic initiation factor 4A-I (eIF-4A-I) (eIF4A-I) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-1), EIF4A1, DDX2A EIF4A |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P60842 |
Иммуноген | Recombinant Human Eukaryotic initiation factor 4A-I protein (167-406AA) |
Источник | Rabbit |
Видовая специфичность | Human, Rat |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
Примечание | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Eukaryotic initiation factor 4A-I antibody at 3µg/ml
Lane 1: K562 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Rat brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47, 40 kDa
Observed band size: 47 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA007550ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA007550ESR1HU at dilution of 1:100
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