| Объем | 50 мкг |
| Синонимы | Golgi phosphoprotein 3 (Coat protein GPP34) (Mitochondrial DNA absence factor) (MIDAS), GOLPH3, GPP34 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H4A6 |
| Иммуноген | Recombinant Human Golgi phosphoprotein 3 protein (16-150AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Golgi phosphoprotein 3 |
| Области исследований | Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
All lanes: GOLPH3 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA872494LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA872494LA01HU at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA872494LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating GOLPH3 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA872494LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA872494LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: GOLPH3 Antibody.