| Объем | 50 мкл |
| Синонимы | Ectonucleoside triphosphate diphosphohydrolase 5 (NTPDase 5) (EC 3.6.1.6) (CD39 antigen-like 4) (ER-UDPase) (Guanosine-diphosphatase ENTPD5) (GDPase ENTPD5) (EC 3.6.1.42) (Nucleoside diphosphatase) (Uridine-diphosphatase ENTPD5) (UDPase ENTPD5), ENTPD5, CD39L4 PCPH |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O75356 |
| Иммуноген | Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 5 protein (149-428AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ENTPD5 antibody at 4µg/ml
Lane 1: Mouse liver tissue
Lane 2: Mouse kidney tissue
Lane 3: Mouse gonad tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
 |
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA007694ESR2HU at dilution of 1:100
 |
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA007694ESR2HU at dilution of 1:100
 |
Immunoprecipitating ENTPD5 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA007694ESR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA007694ESR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
 |
| | |
Наименование: ENTPD5 Antibody.