Объем | 100 мкл |
Синонимы | ER, ESR, ESR1, ESTR, ESTRA |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P03372 |
Иммуноген | Peptide sequence around aa.165~169 (L-A-S-T-N) derived from Human Estrogen Receptor-a. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
Примечание | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.<br/><br/>H Yamaguchi, J Zhu, T Yu, et al. (2007) Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their differentiation state. Cell Biology International, 31(6):638-644.<br/>This article references the use of the #21068 in the following applications :IF-IC<br/> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MCF cells using Estrogen Receptor-a(Ab-167) Antibody and the same antibody preincubated with blocking peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-167) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Ab-167) Antibody.
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