| Объем | 50 мкг |
| Синонимы | Histone H3.3, H3F3A, H3F3B, H3.3A H3F3, H3.3B |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P84243 |
| Иммуноген | Recombinant Human Histone H3.3 protein (2-136AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Histone H3.3 antibody at 2µg/ml
Lane 1: 293T whole cell lysate
Lane 2: EC109 whlole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA01435A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA01435A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA01435A0Rb at dilution of 1:100
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Наименование: H3F3A Antibody.