| Объем | 100 мкг |
| Синонимы | Hypoxia-inducible factor 1-alpha inhibitor (EC 1.14.11.30) (EC 1.14.11.n4) (Factor inhibiting HIF-1) (FIH-1) (Hypoxia-inducible factor asparagine hydroxylase), HIF1AN, FIH1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9NWT6 |
| Иммуноген | Recombinant Human Hypoxia-inducible factor 1-alpha inhibitor protein (2-254AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: HIF1AN antibody at 12µg/ml
Lane 1: Mouse heart tissue
Lane 2: Mouse skeletal muscle tissue
Lane 3: K562 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: HL60 whole cell lysate
Lane 6: 293T whole cell lysate
Lane 7: Jurkat whole cell lysate
Lane 8: A375 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40, 48 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA865160LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA865160LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating HIF1AN in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA865160LA01HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA865160LA01HU (8µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (10µg)
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Immunofluorescent analysis of MCF-7 cells using CSB-PA865160LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: HIF1AN Antibody.