| Объем | 100 мкл |
| Синонимы | Histone H2B type 1-B (Histone H2B.1) (Histone H2B.f) (H2B/f), HIST1H2BB, H2BFF |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P33778 |
| Иммуноген | Peptide sequence around site of Lys (16) derived from Human Histone H2B type 1-B. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IF, ChIP, Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200 |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2B type 1-B |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela cell acid extracts, Jurkat cell acid extracts, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue
All lanes: HIST1H2BB antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
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Immunofluorescent analysis of Hela cells using CSB-PA010402OA16nacHU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2BB (CSB-PA010402OA16nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Наименование: HIST1H2BB (Ab-16) Antibody.