| Объем | 50 мкг |
| Синонимы | Forkhead box protein D3 (HNF3/FH transcription factor genesis), FOXD3, HFH2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UJU5 |
| Иммуноген | Recombinant Human Forkhead box protein D3 protein (1-140AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Forkhead box protein D3 |
| Области исследований | Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Developmental biology, Metabolism, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat kidney tissue
All lanes: FOXD3 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
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Immunohistochemistry of paraffin-embedded human glioma using CSB-PA890729LA01HU at dilution of 1:100
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Immunofluorescence staining of Hela cells with CSB-PA890729LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: FOXD3 Antibody.