| Объем | 50 мкг |
| Синонимы | Forkhead box protein P1 (Mac-1-regulated forkhead) (MFH), FOXP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H334 |
| Иммуноген | Recombinant Human Forkhead box protein P1 protein (1-114AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, ChIP, Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
| Примечание | Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate
All lanes: FOXP1 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 55, 66, 75, 13, 67, 68 kDa
Observed band size: 85 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA008841EA01HU at dilution of 1:100
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Immunofluorescence staining of Hela cells with CSB-PA008841EA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-FOXP1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP008841HU) against the HSP90B promoter.
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Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
All lanes: FOXP1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 55, 66, 75, 13, 67, 78 kDa
Observed band size: 76 kDa
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Наименование: FOXP1 Antibody.