IDH3B AntibodyСпецификацияОбъем | 50 мкл | Синонимы | isocitrate dehydrogenase 3 (NAD+) beta | Клональность | Polyclonal Antibody | Организм | Human | uniprot | O43837 | Иммуноген | Fusion protein of human IDH3B | Источник | Rabbit | Видовая специфичность | Human, Mouse, Rat | Применение | ELISA, WB, IHC,ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 | Примечание | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Antigen affinity purification | Области исследований | Cancer,Metabolism,Signal transduction | Ссылка на страницу на сайте производителя | ссылка | The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA597290(IDH3B Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ?200)
| Gel: 10%SDS-PAGE, Lysate: 40 ?g, Lane: Mouse eyes tissue, Primary antibody: CSB-PA597290(IDH3B Antibody) at dilution 1/240, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
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