| Объем | 100 мкг |
| Синонимы | Mitochondrial fission 1 protein (FIS1 homolog) (hFis1) (Tetratricopeptide repeat protein 11) (TPR repeat protein 11), FIS1, TTC11 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9Y3D6 |
| Иммуноген | Recombinant Human Mitochondrial fission 1 protein (1-122AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:50-1:500, IP:1:200-1:2000 |
| Примечание | Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Mitochondrial fission 1 protein |
| Области исследований | Cancer, Cell biology, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Mouse brain tissue, Mouse heart tissue
All lanes: FIS1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
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IHC image of CSB-PA008684LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescent analysis of Hela cells using CSB-PA008684LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating FIS1 in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA008684LA01HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA008684LA01HU (4µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (20µg)
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Наименование: FIS1 Antibody.