FYN (Ab-530) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | EC 2.7.10.2, FYN, kinase Fyn, P59-FYN, SLK | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P06241 | | Иммуноген | Synthesized non-phosphopeptide derived from human Fyn around the phosphorylation site of tyrosine 530 (P-Q-Y(p)-Q-P). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB,WB:1:500-1:3000 | | Примечание | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.<br/><br/>Kawakami T., Mol. Cell. Biol. 6:4195-4201(1986).<br/>Semba K., Proc. Natl. Acad. Sci. U.S.A. 83:5459-5463(1986).<br/>Rigley K., J. Immunol. 154:1136-1145(1995) | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Neuroscience,Cancer,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HUVEC cells, MCF-7 cells and 293 cells, using Fyn (Ab-530) antibody.
 | Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using Fyn (Ab-530) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: FYN (Ab-530) Antibody.