| Объем | 50 мкг |
| Синонимы | Glutaryl-CoA dehydrogenase, mitochondrial (GCD) (EC 1.3.8.6), GCDH |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q92947 |
| Иммуноген | Recombinant Human Glutaryl-CoA dehydrogenase, mitochondrial protein (45-300AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
All lanes: GCDH antibody at 8µg/ml
Lane 1: Mouse kidney tissue
Lane 2: Mouse liver tissue
Lane 3: Hela whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: LO2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49, 48 kDa
Observed band size: 49 kDa
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA849798LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA849798LA01HU at dilution of 1:100
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Immunoprecipitating GCDH in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA849798LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA849798LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: GCDH Antibody.