Объем | 100 мкл |
Синонимы | FERM domain-containing protein 6 (Willin), FRMD6, C14orf31 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q96NE9 |
Иммуноген | Recombinant Human FERM domain-containing protein 6 protein (413-622AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:500 |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | FERM domain-containing protein 6 |
Области исследований | Cancer |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA822270ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human duodenal tissue using CSB-PA822270ESR2HU at dilution of 1:100
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Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
All lanes: FRMD6 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73, 71, 30 kDa
Observed band size: 73 kDa
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IHC image of CSB-PA822270ESR2HU diluted at 1:293 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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