| Объем | 100 мкг |
| Code NO# | 1C91F1 |
| Синонимы | Glial fibrillary acidic protein, GFAP |
| Тип антител | Monoclonal Antibody |
| Видовая специфичность | Human |
| Uniport ID | P14136 |
| Иммуноген | Recombinant Human GFAP protein (292-432AA) |
| Источник | Mouse |
| Видовая специфичность 1 | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, FC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200 |
| Примечание | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Изотип | IgG2b |
| Метод очистки | >95%, Protein G purified |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Области исследований | Neuroscience, Tags & Cell Markers, Signal transduction, Stem cells |
| Станица на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue, Rat brain tissue
All lanes: GFAP antibody at 2.7µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-MA009369A0m at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human glioma using CSB-MA009369A0m at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA009369A0m at dilution of 1:100
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Immunofluorescent analysis of A549 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescent analysis of SH-SY5Y cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescent analysis of U251 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescent analysis of U87 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Overlay histogram showing SH-SY5Y cells stained with CSB-MA009369A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG2b (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Наименование: GFAP Monoclonal Antibody.