| Объем | 100 мкг |
| Клон ID | 4D8C10 |
| Синонимы | CFP, eGFP, eYFP, GFP, GFP tag, YFP |
| Тип антител | Tag/Control Antibodies |
| Иммуноген | Recombinant GFP Protein |
| Источник | Mouse |
| Применение | ELISA,WB,IP,Recommended dilution:WB:1:500-1:5000,IP:1:200-1:2000 |
| Изотип | IgG2b |
| Коньюгат | Non-conjugated |
| Метод очистки | >95%,Protein G purified |
| Буффер | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Примечание | The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. |
| Ссылка на страницу на сайте производителя | ссылка |
WB: Mouse Anti-GFP monoclonal antibody at 0.1ug/ml
Lane 1:HEK-293 cell lysate
Lane 2:GFP transfected HEK-293 cell lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd
 |
IP:Result of anti-GFP-tag Monoclonal antibody
Line 1:Control IgG
Line 2:Precipitateing GFP-tagged fusion protein
Line 3:GFP Transfected HEK-293 cells lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd
 |
| | |
Наименование: GFP Monoclonal Antibody.