GAPDH Monoclonal AntibodyСпецификация| Объем | 50 мкл | | Клон ID | 14C2F11 | | Синонимы | GAPDH, G3PD, GAPD, MGC88685 | | Тип антител | Tag/Control Antibodies | | Иммуноген | Recombinant Human GAPDH protein | | Источник | Mouse | | Видовая специфичность | All | | Применение | ELISA, WB, IHC, IP, IF, FC, Recommended dilution: WB:1:2000-1:10000, IHC:1:50-1:500, IF:1:50-1:200, IP:1:2000-1:5000 | | Изотип | IgG1 | | Коньюгат | Non-conjugated | | Метод очистки | >95%, Protein G purified | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Примечание | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it, third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1’s nuclear localization signal, and degrades Siah1’s substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. (?)-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: 15?g hela whole cell lysate
GAPDH antibody at 1:10W, 1:20W, 1:40W, 1:80W, 1:160W
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min
 | Western Blot
Positive WB detected in: Hela whole cell lysate at 10?g, 5?g, 2.5?g, 1.25?g, 0.625?g, 0.3125?g
All lanes: GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min
 | Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 30s
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Информация для заказа |
Наименование: GAPDH Monoclonal Antibody.