GBA AntibodyСпецификацияОбъем | 50 мкг | Синонимы | Glucosylceramidase (EC 3.2.1.45) (Acid beta-glucosidase) (Alglucerase) (Beta-glucocerebrosidase) (Beta-GC) (D-glucosyl-N-acylsphingosine glucohydrolase) (Imiglucerase), GBA, GC GLUC | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P04062 | Иммуноген | Recombinant Human Glucosylceramidase protein (215-343AA) | Источник | Rabbit | Видовая специфичность | Human | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000 | Примечание | extracellular exosome, lysosomal lumen, lysosomal membrane, glucosylceramidase activity, receptor binding, cellular response to tumor necrosis factor, ceramide biosynthetic process, glucosylceramide catabolic process, glycosphingolipid metabolic process, negative regulation of inflammatory response | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | >95%, Protein G purified | Абревеатура | Glucosylceramidase | Области исследований | Neuroscience, Cancer, Metabolism, Signal transduction | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate
All lanes: GBA antibody at 3.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 58, 30, 51, 55 kDa
Observed band size: 51 kDa
| IHC image of CSB-PA009289LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
| | |
Информация для заказа |