Объем | 50 мкл |
Синонимы | Golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, C9orf155, GOLPH2, PSEC0242, UNQ686/PRO1326 |
Тип антител | Recombinant Antibody |
Species | Human |
UniProt ID | Q8NBJ4 |
Иммуноген | A synthesized peptide derived from human GOLM1 |
Видовая специфичность | Human |
Применение | ELISA, WB, FC, Recommended dilution: WB:1:500-1:5000 |
Клональность | Monoclonal |
Изотип | Rabbit IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Affinity-chromatography |
Области исследований | Signal Transduction |
Аббревиатура | Golgi membrane protein 1 |
Примечание | Unknown. Cellular response protein to viral infection. |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate
All lanes: GOLM1 antibody at 1.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 45 KDa
Observed band size: 80 KDa
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Overlay histogram showing Hela cells stained with CSB-RA009666A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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