Объем | 100 мкг |
Синонимы | G protein-coupled receptor kinase 4 (EC 2.7.11.16) (G protein-coupled receptor kinase GRK4) (ITI1), GRK4, GPRK2L GPRK4 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P32298 |
Иммуноген | Recombinant Human G protein-coupled receptor kinase 4 protein (1-245AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin, the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: GRK4 antibody at 2µg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 67, 64, 58, 62 kDa
Observed band size: 67 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009925LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA009925LA01HU at dilution of 1:100
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Immunofluorescent analysis of PC-3 cells using CSB-PA009925LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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