| Объем | 100 мкг |
| Синонимы | Gelsolin (AGEL) (Actin-depolymerizing factor) (ADF) (Brevin), GSN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P06396 |
| Иммуноген | Recombinant Human Gelsolin protein (28-782AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Gelsolin antibody at 2µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 86, 81, 82 kDa
Observed band size: 86 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA009965HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA009965HA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA009965HA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA009965HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: THP-1 whole cell lysate
All lanes: GSN antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86, 81, 82 kDa
Observed band size: 86 kDa
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IHC image of CSB-PA009965HA01HU diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: GSN Antibody.