Объем | 50 мкг |
Синонимы | Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (EC 4.2.1.134) (3-hydroxyacyl-CoA dehydratase 2) (HACD2) (Protein-tyrosine phosphatase-like member B), HACD2, PTPLB |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q6Y1H2 |
Иммуноген | Recombinant Human Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 protein (7-41AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cardiovascular, Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: HACD2 antibody at 4µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: LO2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA761567LA01HU at dilution of 1:100
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Immunofluorescent analysis of MCF-7 cells using CSB-PA761567LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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