| Объем | 100 мкл |
| Синонимы | RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) [Includes: RNA-directed RNA polymerase (EC 2.7.7.48), mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56), GDP polyribonucleotidyltransferase (EC 2.7.7.88), Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC 2.1.1.296)], L |
| Клональность | Polyclonal Antibody |
| Организм | Vesicular stomatitis Indiana virus |
| uniprot | Q8B0H0 |
| Иммуноген | Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L protein (598-784AA) |
| Источник | Rabbit |
| Видовая специфичность | Vesicular stomatitis Indiana virus |
| Применение | ELISA, WB, Recommended dilution: WB:1:500-1:5000 |
| Примечание | RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | RNA-directed RNA polymerase L |
| Области исследований | N/A |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in Recombinant protein
All lanes: RNA-directed RNA polymerase L antibody at 7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
 |
| | |
Наименование: L Antibody.