| Объем | 100 мкл |
| Синонимы | Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P16403 |
| Иммуноген | Peptide sequence around site of Lys (186) derived from Human Histone H1.2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IP, Recommended dilution: WB:1:100-1:1000, IP:1:200-1:2000 |
| Примечание | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H1.2 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate
All lanes: HIST1H1C antibody at 1:100
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
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Immunoprecipitating HIST1H1C in 293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010378PA186nme1HU in 293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010378PA186nme1HU (5µg) + 293 whole cell lysate (500µg)
Lane 3: 293 whole cell lysate (20µg)
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Наименование: HIST1H1C (Ab-186) Antibody.