| Объем | 100 мкл |
| Синонимы | Histidine--tRNA ligase, cytoplasmic (EC 6.1.1.21) (Histidyl-tRNA synthetase) (HisRS), HARS, HRS |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P12081 |
| Иммуноген | Recombinant Human Histidine--tRNA ligase, cytoplasmic protein (1-180AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: HARS antibody at 6µg/ml
Lane 1: Hela whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 58, 53, 51, 55 kDa
Observed band size: 58 kDa
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Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA010137ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA010137ESR2HU at dilution of 1:100
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Immunoprecipitating HARS in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA010137ESR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA010137ESR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: HARS Antibody.