HIST1H3A (Ab-14) Antibody

Спецификация

Объем	100 мкл
Синонимы	Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ
Клональность	Polyclonal Antibody
Организм	Human
uniprot	P68431
Иммуноген	Peptide sequence around site of Lys (14) derived from Human Histone H3.1.
Источник	Rabbit
Видовая специфичность	Human, Mouse, Rat
Применение	ELISA, WB, IHC, IF, ChIP, Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Примечание	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Клональность1	Polyclonal
Изотип	IgG
Коньюгат	Non-conjugated
Буффер	Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Форма	Liquid
Хранение	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки	Antigen Affinity Purified
Абревеатура	Histone H3.1
Области исследований	Epigenetics and Nuclear Signaling
Ссылка на страницу на сайте производителя	ссылка
Western Blot Positive WB detected in: Jurkat cell acid extracts, HEK293 cell acid extracts, Mouse brain tissue All lanes: HIST1H3A antibody at 1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010418PA14nacHU at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA010418PA14nacHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H3A (CSB-PA010418PA14nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.

Информация для заказа

Область использования: Поликлональные антитела Cusabio	Производство:	Cusabio
	Метод:	Антитела
	Объем:	100 мкл
	Кат. номер:	CSB-PA010418PA14nacHU
Цена (с НДС 20%):	по запросу	В корзину
Наименование: HIST1H3A (Ab-14) Antibody. Примечание: дополнительная информация (на английском языке).