| Объем | 50 мкг |
| Синонимы | LexA repressor (EC 3.4.21.88), lexA, exrA spr tsl umuA |
| Клональность | Polyclonal Antibody |
| Организм | Escherichia coli |
| uniprot | P0A7C2 |
| Иммуноген | Recombinant Escherichia coli LexA repressor protein (1-202AA) |
| Источник | Rabbit |
| Видовая специфичность | Escherichia coli |
| Применение | ELISA, WB, Recommended dilution: WB:1:500-1:5000 |
| Примечание | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | LexA repressor |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in Recombinant protein
All lanes: lexA antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
predicted band size: 39 kDa
observed band size: 39 kDa
 |
| | |
Наименование: lexA Antibody.