| Объем | 100 мкл |
| Синонимы | Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P68431 |
| Иммуноген | Peptide sequence around site of Lys (9) derived from Human Histone H3.1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, ChIP, Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H3.1 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela cell acid extracts, A549 cell acid extracts, PC-3 cell acid extracts, HEK293 cell acid extracts
All lanes: HIST1H3A antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010418OA09nme1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010418OA09nme1HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA010418OA09nme1HU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H3A (CSB-PA0104180A09nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Наименование: HIST1H3A (Ab-9) Antibody.