HMGB1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | high mobility group box 1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P09429 | | Иммуноген | Synthetic peptide of human HMGB1 | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 | | Примечание | High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NF B family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen affinity purification | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA225274(HMGB1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ?200)
 | Gel: 10%SDS-PAGE, Lysate: 40 ?g, Lane 1-5: Human hepatocellular carcinoma tissue, Hela cells, Jurkat cells, 293T cells, human breast infiltrative duct tissue, Primary antibody: CSB-PA225274(HMGB1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
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Наименование: HMGB1 Antibody.