| Объем | 50 мкл |
| Синонимы | Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG, HIST1H2AI, HIST1H2AK, HIST1H2AL, HIST1H2AM, H2AFP, H2AFC, H2AFD, H2AFI, H2AFN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P0C0S8 |
| Иммуноген | Peptide sequence around site of Mono-methyl-Lys (118) derived from Human Histone H2A |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IP, ChIP, Recommended dilution: WB:1:200-1:2000, IP:1:200-1:2000 |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2A type 1 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes: HIST1H2AG antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
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Immunoprecipitating HIST1H2AG in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010389OA118nme1HU in NIH/3T3 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010389OA118nme1HU (5µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg)
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Chromatin Immunoprecipitation Hela (106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2AG (CSB-PA010389OA118nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Наименование: HIST1H2AG (Ab-118) Antibody.