HIST1H2BC (Ab-12) AntibodyСпецификацияОбъем | 100 мкл | Синонимы | Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC, HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI, H2BFL, H2BFH, H2BFG, H2BFA, H2BFK | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P62807 | Иммуноген | Peptide sequence around site of Mono-methyl-Lys (12) derived from Human Histone H2B | Источник | Rabbit | Видовая специфичность | Human, Mouse, Rat | Применение | ELISA, WB, ChIP, Recommended dilution: WB:1:200-1:2000 | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Antigen Affinity Purified | Абревеатура | Histone H2B type 1-C/E/F/G/I | Области исследований | Epigenetics and Nuclear Signaling | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat liver tissue, Mouse kidney tissue
All lanes: HIST1H2BC antibody at 1.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
| Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA12nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Информация для заказаОбласть использования: | Производство: | Cusabio | Метод: | Антитела | Объем: | 100 мкл | Кат. номер: | CSB-PA010403OA12nme1HU | Цена (с НДС 20%): | по запросу | В корзину | Наименование: HIST1H2BC (Ab-12) Antibody. Примечание: дополнительная информация (на английском языке). |
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