| Объем | 50 мкл |
| Синонимы | Histone deacetylase 6, HD6, HDAC6, KIAA0901, JM21 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | Q9UBN7 |
| Иммуноген | A synthesized peptide derived from human HDAC6 |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Epigenetics and Nuclear Signaling |
| Аббревиатура | Histone deacetylase 6 |
| Примечание | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. |
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Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
All lanes: HDAC6 antibody at 1.1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 132, 115 KDa
Observed band size: 160 KDa
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IHC image of CSB-RA010242A0HU diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating HDAC6 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA010242A0HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA010242A0HU (3µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)
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Наименование: HDAC6 Antibody.