Объем | 50 мкг |
Синонимы | Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Lung cancer antigen NY-LU-1) (Muscle-type aldolase), ALDOA, ALDA |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P04075 |
Иммуноген | Recombinant Human Fructose-bisphosphate aldolase A protein (2-364AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein .Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881], also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.Belongs to the class I fructose-bisphosphate aldolase family. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Fructose-bisphosphate aldolase A protein |
Области исследований | Cancer, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Fructose-bisphosphate aldolase A polyclonal antibody at 2µg/ml + EC109 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 40, 46 kDa
Observed band size: 40 kDa
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Immunohistochemistry of paraffin-embedded Mouse heart cancer using CSB-PA00015A0Rb at dilution of 1:50
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IHC image of CSB-PA00015A0Rb diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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