Объем | 50 мкг |
Синонимы | Interferon-induced protein with tetratricopeptide repeats 5 (IFIT-5) (Interferon-induced 58 kDa protein) (Retinoic acid- and interferon-inducible 58 kDa protein) (P58), IFIT5, ISG58 RI58 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q13325 |
Иммуноген | Recombinant Human Interferon-induced protein with tetratricopeptide repeats 5 protein (107-320AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and binds tRNAs. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: IFIT5 antibody at 2µg/ml
Lane 1: A375 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 56, 51 kDa
Observed band size: 56 kDa
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Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA011023LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA011023LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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