MAP2K1 (Ab-221) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q02750 | | Иммуноген | Peptide sequence around aa.219~223 (A-N-S-F-V) derived from Human MEK1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.<br/><br/>Zebisch A, et al. (2006) Cancer Res, 66(7): 3401-8.<br/>Luciano BS, et al. (2006)J Biol Chem, 279(50): 52117-23.<br/>Wang X, et al. (2003) Oncogene, 22(1): 109-16.<br/>Gopalbhai K, et al. (2003) J Biol Chem, 278(10): 8118-25.<br/>Ling MT, et al. (2002)Oncogene, 21(55): 8498-505. <br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 cells using MEK1(Ab-221) Antibody
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1(Ab-221) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: MAP2K1 (Ab-221) Antibody.