| Объем | 50 мкг |
| Синонимы | Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) (Helix-destabilizing protein) (Single-strand RNA-binding protein) (hnRNP core protein A1) [Cleaved into: Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed], HNRNPA1, HNRPA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P09651 |
| Иммуноген | Recombinant Human Heterogeneous nuclear ribonucleoprotein A1 protein (2-354AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Microbiology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: HNRNPA1 antibody at 6µg/ml
Lane 1: K562 whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39, 35, 30 kDa
Observed band size: 39 kDa
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Immunohistochemistry of paraffin-embedded Mouse heart tissue using CSB-PA010600HA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA010600HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: HNRNPA1 antibody at 3.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 35, 30 kDa
Observed band size: 39 kDa
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IHC image of CSB-PA010600HA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: HNRNPA1 Antibody.