Объем | 50 мкл |
Синонимы | DNA replication licensing factor MCM5 (EC 3.6.4.12) (CDC46 homolog) (P1-CDC46), MCM5, CDC46 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P33992 |
Иммуноген | Recombinant Human DNA replication licensing factor MCM5 protein (1-230AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
Примечание | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: DNA replication licensing factor MCM5 antibody at 3µg/ml
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 2: Mouse thymus tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013595ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA013595ESR1HU at dilution of 1:100
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