| Объем | 50 мкг |
| Синонимы | Integrin-linked protein kinase (EC 2.7.11.1) (59 kDa serine/threonine-protein kinase) (ILK-1) (ILK-2) (p59ILK), ILK, ILK1 ILK2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13418 |
| Иммуноген | Recombinant Human Integrin-linked protein kinase protein (1-228AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat, Zebrafish |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Integrin-linked protein kinase protein |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ILK antibody at 3µg/ml
Lane 1: Hela whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: HL60 whole cell lysate
Lane 4: Mouse liver tissue
Lane 5: Mouse lung tissue
Lane 6: Rat liver tissue
Lane 7: Rat lung tissue
Lane 8: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52, 45, 37 kDa
Observed band size: 52, 37 kDa
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Immunohistochemistry of paraffin-embedded 293T cells using CSB-PA00999A0Rb at dilution of 1:100 Immunohistochemistry
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00999A0Rb at dilution of 1:20
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Immunofluorescent analysis of Hela cells using CSB-PA00999A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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IHC image of CSB-PA00999A0Rb diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of MCF-7 cells with CSB-PA00999A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: ILK Antibody.