| Объем | 100 мкг |
| Синонимы | Interferon regulatory factor 3 (IRF-3), IRF3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14653 |
| Иммуноген | Recombinant Human Interferon regulatory factor 3 protein (1-191AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Interferon regulatory factor 3 |
| Области исследований | Epigenetics and Nuclear Signaling, Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: THP-1 whole cell lysate, A549 whole cell lysate
All lanes: IRF3 antibody at 5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 34, 17, 50, 13 kDa
Observed band size: 48 kDa
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Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA011818LA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA011818LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: IRF3 Antibody.