| Объем | 50 мкг |
| Синонимы | Endoplasmin (94 kDa glucose-regulated protein) (GRP-94) (Heat shock protein 90 kDa beta member 1) (Tumor rejection antigen 1) (gp96 homolog), HSP90B1, GRP94 TRA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P14625 |
| Иммуноген | Recombinant Human Endoplasmin protein (27-799AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors By similarity. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Endoplasmin antibody at 2µg/ml
Lane 1: A549 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDa
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Western blot
All lanes: HSP90B1 antibody at 4.5 µg/ml + HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDa
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA10887A0Rb at dilution of 1:100
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Western Blot
Positive WB detected in: MCF-7 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
All lanes: HSP90B1 antibody at 6.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
 |
Immunofluorescence staining of Hela cells with CSB-PA10887A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: HSP90B1 Antibody.