| Объем | 50 мкл |
| Синонимы | Heat shock-related 70 kDa protein 2 (Heat shock 70 kDa protein 2), HSPA2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P54652 |
| Иммуноген | Recombinant Human Heat shock-related 70 kDa protein 2 protein (400-639AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cancer, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: HSPA2 antibody at 6µg/ml
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Rat brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010824ESR2HU at dilution of 1:100
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Immunoprecipitating HSPA2 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA010824ESR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA010824ESR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: HSPA2 Antibody.