Объем | 100 мкл |
Синонимы | mitochondrial 28S ribosomal protein S22, MRP-S22, RPMS22, RT22, S22mt |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P82650 |
Иммуноген | Synthesized peptide derived from internal of human MRPS22. |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF,WB:1:500-1:3000, IF:1:100-1:500 |
Примечание | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq.<br/><br/>Crisponi L., Nat. Genet. 27:159-166(2001).<br/>Li Y., Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.<br/>The MGC Project Team, Genome Res. 14:2121-2127(2004).<br/> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from COS cells, using MRPS22 antibody.
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Immunofluorescence analysis of A549 cells, using MRPS22 antibody.
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