| Объем | 100 мкл |
| Синонимы | Interleukin-1 receptor-associated kinase 4 (IRAK-4) (EC 2.7.11.1) (Renal carcinoma antigen NY-REN-64), IRAK4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9NWZ3 |
| Иммуноген | Recombinant Human Interleukin-1 receptor-associated kinase 4 protein (1-220AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IP, Recommended dilution: WB:1:500-1:2000, IP:1:200-1:2000, IP:1:200-1:2000 |
| Примечание | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cardiovascular, Immunology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Interleukin-1 receptor-associated kinase 4 antibody at 4µg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52, 38 kDa
Observed band size: 52 kDa
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Immunoprecipitating IRAK4 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA011812ESR1HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA011812ESR1HU (8µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (10µg)
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Наименование: IRAK4 Antibody.