Объем | 50 мкг |
Синонимы | GTPase KRas (K-Ras 2) (Ki-Ras) (c-K-ras) (c-Ki-ras) [Cleaved into: GTPase KRas, N-terminally processed], KRAS, KRAS2 RASK2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P01116 |
Иммуноген | Recombinant Human GTPase KRas protein (2-168AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | GTPase KRas protein |
Области исследований | Epigenetics and Nuclear Signaling, Cancer, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA13969A0Rb at dilution of 1:100
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Western blot
All lanes: KRAS antibody at 2µg/ml
Lane 1: Mouse brain tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
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Immunofluorescence staining of Hela cells with CSB-PA13969A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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