| Объем | 50 мкг |
| Синонимы | Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) (Type-II keratin Kb8), KRT8, CYK8 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P05787 |
| Иммуноген | Recombinant Human Keratin, type II cytoskeletal 8 protein (19-148AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Zebrafish |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: KRT8 antibody at 2µg/ml
Lane 1: A549 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54, 57 kDa
Observed band size: 54 kDa
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Western blot
All lanes: KRT8 antibody at 8µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: Zebrafish lysate
eSecondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54, 57 kDa
Observed band size: 54 kDa
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA11269A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA11269A0Rb at dilution of 1:100
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Immunofluorescence staining of Hela cells with CSB-PA11269A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: KRT8 Antibody.