| Объем | 50 мкг |
| Синонимы | Plastin-2 (L-plastin) (LC64P) (Lymphocyte cytosolic protein 1) (LCP-1), LCP1, PLS2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P13796 |
| Иммуноген | Recombinant Human Plastin-2 protein (2-627AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IP:1:200-1:2000 |
| Примечание | Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Plastin-2 |
| Области исследований | Cancer, Immunology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Plastin-2 antibody at 2µg/ml
Lane 1: HL60 whole cell lysate
Lane 2: THP-1 whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: NIH/3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 71, 22 kDa
Observed band size: 71 kDa
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IHC image of CSB-PA012824HA01HU diluted at 1:400 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating LCP1 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA012824HA01HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA012824HA01HU (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)
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Наименование: LCP1 Antibody.